Analysis Note

ControlPositive Control Included: Non-Stim A431 Cell Lysate (12-301)

Application

Detect SHP-2/SHPTP-2 using this Anti-SHP-2/SHPTP-2 Antibody validated for use in IC & WB.

Immunocytochemistry Analysis: 4.0 µg/mL from a representative lot detected SHP-2/SHPTP-2 in HeLa, A431, HUVEC, and NIH/3T3 cells.

Research CategorySignaling

Research Sub CategoryCytoskeletal Signaling

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Tyrosine-protein phosphatase non-receptor type 11 (EC 3.1.3.48; UniProt P35235; also known as Protein-tyrosine phosphatase SYP; SH-PTP2, SH2 domain-containing protein tyrosine phosphatase-2, SHP-2, Shp2) is encoded by the Ptpn11 (also known as Syp) gene (Gene ID 19247) in murine species. SHP-2/SHPTP-2 is a non-receptor protein tyrosine phosphatase involved in a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. SHP-2/SHPTP-2 contains two tandem Src homology-2 (SH2) domains that mediate substrates interaction, followed by a protein tyrosine phosphatase PTP domain. Peptide library-based study reveals similar substrate specificities of SHP-2 and the related SHP-1. Both enzymes exhibit preference for acidic and hydrophobic residues flanking the N- and C-terminal sides of target tyrosine residue. Mutations in PTPN11 gene are known to cause Noonan syndrome and acute myeloid leukemia in human.

Immunogen

GST-fusion protein containing residues 2-216 of mouse SHP-2/SHPTP-2

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein A purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected SHP-2/SHPTP-2 in 10 µg of HeLa cell lysate.

Specificity

Recognizes SHP-2/SHPTP-2.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

64 kDa, apparent size increases to approximately 70 kDa upon tyrosine phosphorylation.